• Extraction and estimation of oil for seed & other material by solvent extraction method
• Trans-esterification of seed oil at lab scale
• Biodiesel production at lab scale
• Estimation of physicochemical properties of oil / biodiesel
• Physico-chemical comparative study between commercial diesel and biodiesel

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• Collection, Isolation, and identification of microalgae and cyanobacteria
• Media preparation, Inoculation and preservation of microalgae
• Media preparation and sterilization techniques
• Biochemical characterization of microalgae and cyanobacteria
• Conservation and mass multiplication of elite plant sourceCultivation of microalgae in various medium using wastewater and other medium
• Phytoremediation/ Bioremediation
• Characterization of microalgae and cyanobacteria for high-value compounds/ molecules
• Lipid profile analysis of microalgae

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• Basics of Biofertilisers and Biopesticides
• Production of biofertilisers and biopesticides.
• Quality testing of biofertilisers and biopesticides
• In-vitro & In-Vivo testing of Bio-efficacy of Biofertilisers & Biopesticides.
• Storage and self life of Biofertilisers and Biopesticides

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• Introduction and Understanding of Bioinformatics: Application, Scope, and Importance of Bioinformatics
• Introduction of different Bioinformatics tools and their applications
• Biological databases: Primary and secondary databases use and applications in in silico analysis
• Sequence Databases similarity searching tools: FASTA, BLAST (BLASTP, BLASTN, BLASTX, etc.), Genome and Proteome analysis
• Introduction to Computer-Aided Drug Design, Virtual Screening and its application, Molecular Docking, Molecular Dynamics, Pharmacokinetic analysis (ADME/T), Toxicity assessment
• Machine learning: Applications in Drug Design: Regression Analysis, Multiple Linear Regression, Algorithm etc.
• Phylogenetic analysis and its application, Protein structure prediction, and validation, classification, and family databases (PDB, Swiss-Prot, etc.
• Experimental dataset Collection/compilation of biological data, Computational library preparation biological for designed in silico analysis
• Structure-Activity analysis/ Modelling using R / Python programming and chemical structure designing
• AI-based applications in Drug Design and Development

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• Post-harvest processing for higher yield of plant secondary metabolites
• Sample collection and preparation
• Solid-liquid extraction
• Purification techniques by column chromatography column chromatography
• Phytochemical analysis
• Bioactivity of partially purified phytocompounds

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• Introduction to Industrial Biotechnology: Scope, Traditional and Modern Biotechnology: A brief survey of processes and products
• Culturing and sub-culturing of microbes and scale-up of cultures
• Media optimization: Plackett Burman design
• Basics of bioreactor and its parts, types of bioreactors.
• Concept of batch/fed-batch/continuous mode of operations
• Cell Conventional techniques of media optimization (one factor at a time: OFAT) techniques
• Advanced techniques for media optimization (Plackett Burman Design/ Central Composite Design, Response Surface Methodology/ Artificial Neural Network)
• Types of fermentation (solid state/ submerged)
• Cell disruption techniques
• Cell Harvesting Techniques
• Extraction: Aqueous two phase extraction, precipitation
• Product purification: Chromatography example: HPLC, GC, TLC
• Entrepreneurship concept: Entrepreneurship as a career

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• Overview of molecular biology techniques and their application.
• Structural and functional aspects of various biomolecules (nucleic acid and protein)
• Different techniques/methods of extraction and purification of biomolecules from different samples (plant, microbes, etc.)
• Plasmid extraction and purification.
• Quantification of isolated biomolecules with different techniques (agarose gel electrophoresis, Spectrophotometery)
• Gel documentation by GELDOCs system with advanced software
• Polymerase Chain Reaction (PCR): principle, procedure and product purification
• Plant DNA fingerprinting

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• Plant tissue culture: Technique and application
• Record maintenance in tissue material and culture laboratories
• Media preparation and sterilization techniques
• Explant selection and micropropagation techniques
• Conservation and mass multiplication of elite plant sources
• Molecular characterization of tissue cultured plants.
• Grading packing and dispatch
• Standardization of equipment and fumigation of lab and materials
• Record maintenance and accreditation

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• Determination procedure of physical parameters of water quality testing
• Determination procedure of chemical parameters of water quality testing
• Microbiological analysis procedure of water and wastewater samples
• Role of microbes in the removal of heavy metal ions/oils/dyes/organic pollutants
• Determination of maximum resistance level (MRL) of different microbial strains against heavy metals (like Zn, Cu, Cr, Mn, Pb, Cd, Ni, Fe, etc)/ oils/dyes/organic pollutants.
• Quantitative estimation of biosorption/ bio-adsorption of heavy metal by bacteria and fungi
• Example of the treatment process for wastewater of dairy, tannery, sugar and textile industry
• Application of biodegradation/bioremediation techniques for industrial effluents and wastewater by native and external microbes

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• Isolation and Identification of Food Microbes
• Fermentation Process and Yeast Propagation
• Production of Yogurt Using Probiotic Bacteria
• Microbial Analysis of Food Spoilage
• Detection of Foodborne Pathogens
• Antimicrobial Activity of Natural Extracts
• Application of natural extracts to reduce Food Spoilage

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